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KMID : 0903519830260010041
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1983 Volume.26 No. 1 p.41 ~ p.46
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Studies on Polyphenol Oxidase of Tricholoma matsutake(S . Ito et Imai) Sing
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이태규/Lee, T K
양희천/홍재식/손희숙/Yang, H C/Hong, J S/Sohn, H S
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Abstract
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The characteristics of crude polyphenol oxidase extracted from mushroom[Trichotoma matsutake(S. Ito et Imai) Sing.] were investigated.
The enzyme showed highest affinity to pyrogaroll among trihydroxyphenols. Except for o-diphenols the enzyme was inactive in di- and monophenols. The optimum pH was about 4 and the optimum temperature ranged from 45 to 55℃. The enzyme activity was completely inhibited by 70℃ heat treatment for 2 min. Cu^(++), Fe^(++) and Mg^(++) activated the enzyme at low concentration(10^(-1)mM), but inhibited at high concentration (1mM). The most potent inhibitors were Na-diethyldithiocarbamate, L-ascorbic acid, L-cysteine and NaCl. The Km value with pyrogaroll was 0.88mM.
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